Laboratory Exercises in Microbiology
12th Edition
1264775660
·
9781264775668
© 2023 | Published: February 1, 2022
The Rigel lab is a majors level manual that accompanies our Willey – Prescott’s Microbiology textbook. This laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology…
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- Colour, print bound version of the complete text
- PART ONE
- Microscopic Techniques
- 1. Bright-Field Light Microscopy and Microscopic Measurement of Organisms
- 2. The Hanging Drop Slide and Bacterial Motility
- 3. Dark-Field Light Microscopy
- 4. Phase-Contrast Microscopy
- PART TWO
- Bacterial Cell Biology
- 5. Aseptic Technique
- 6. Negative Staining
- 7. Simple Staining
- 8. Gram Stain
- 9. Acid-Fast Staining Procedure
- 10. Endospore Staining
- 11. Capsule Staining
- 12. Flagella Staining
- PART THREE
- Basic Culture Techniques
- 13. Preparation of Microbiology Media and Equipment
- 14. The Spread-Plate Technique
- 15. The Streak-Plate Technique
- 16. The Pour-Plate Technique
- 17. Cultivation of Anaerobic Bacteria
- 18. Determination of Bacterial Numbers
- PART FOUR
- Microbial Biochemistry
- 19. Carbohydrates I: Fermentation and ?-Galactosidase Activity
- 20. Carbohydrates II: Triple Sugar Iron Agar Test
- 21. Carbohydrates III: Starch Hydrolysis
- 22. Lipid Hydrolysis
- 23. Proteins I: The IMViC Tests
- 24. Proteins II: Gelatin and Casein Hydrolysis
- 25. Proteins III: Catalase Activity
- 26. Proteins IV: Oxidase Test
- 27. Proteins V: Urease Activity
- 28. Proteins VI: Lysine and Ornithine Decarboxylase Tests
- 29. Proteins VII: Phenylalanine Deamination
- 30. Proteins VIII: Dissimilatory Nitrate Reduction
- PART FIVE
- Environmental Factors Affecting Growth of Microorganisms
- 31. Temperature
- 32. pH
- 33. Osmotic Pressure
- 34. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products
- 35. The Effects of Chemical Agents on Bacteria II: Antibiotics
- 36. Bacterial Growth Curve
- PART SIX
- Environmental and Food Microbiolog
- 37. Monitoring Water for Coliforms
- 38. Enumeration of Soil Microorganisms
- 39. Winogradsky Columns
- 40. Bioluminescence
- 41. Plate Counts and Quality Assessment of Milk
- PART SEVEN
- Medical Microbiology
- 42. Staphylococci
- 43. Pneumococcus
- 44. Streptococci
- 45. Neisseriae
- 46. Normal Human Flora
- PART EIGHT
- Eukaryotic Microbiology
- 47. Fungi I: Yeasts (Ascomycota)
- 48. Fungi II: Zygomycota (Rhizopus), Ascomycota (Penicillium), and Basidiomycota (Agaricus)