LooseLeaf Benson's Microbiological Applications Laboratory Manual--Concise Version

Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Microscopic MeasurementsPart 2 Survey of Microorganisms5 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria6 Ubiquity of Bacteria7 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms8 Aseptic Technique9 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms10 Smear Preparation11 Simple Staining12 Negative Staining13 Capsular Staining14 Gram Staining15 Spore Staining: Two Methods16 Acid-Fast Staining: Kinyoun Method17 Motility DeterminationPart 5 Culture Methods18 Culture Media Preparation19 Enumeration of Bacteria: The Standard Plate Count20 Slide Culture: FungiPart 6 Bacterial Viruses21 Determination of a Bacteriophage Titer22 Isolation of Phage from Flies23 Phage TypingPart 7 Environmental Influences and Control of Microbial Growth24 Effects of Oxygen on Growth25 Temperature: Effects on Growth26 pH and Microbial Growth27 Water Activity and Osmotic Pressure28 Ultraviolet Light: Lethal Effects29 The Effects of Lysozyme on Bacterial Cells30 Evaluation of Alcohol: Its Effectiveness as an Antiseptic31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method32 Evaluation of Antiseptics: The Filter Paper Disk Method33 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria34 Morphological Study of An Unknown Bacterium35 Cultural Characteristics36 Physiological Characteristics: Oxidation and Fermentation Stet Reactions37 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions38 Physiological Characteristics: Multiple Test Media39 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems40 Enterobacteriaceae Identification: The API 20E System41 Enterobacteriaceae Identification: The Entero-Pluri Test42 Staphylococcus Identification: The API Staph SystemPart 10 Applied Microbiology43 Bacterial Food Counts44 Bacteriological Examination of Water: Most Probable Number Determination45 Bacteriological Examination of Water: The Membrane Filter Method46 Reductase Test47 Temperature: Lethal Effects48 Microbial Spoilage of Canned Food49 Microbiology of Alcohol FermentationPart 11 Bacterial Genetics and Biotechnology50 Polymerase Chain51 Bacterial TransformationPart 12 Immunology and Serology52 The Staphylococci: Isolation and Identification53 The Streptococci and Enterococci: Isolation and Identification54 Gram-Negative Intestinal Pathogens55 A Synthetic EpidemicPart 13 Immunology and Serology56 Slide Agglutination Test: Serological Typing57 Slide Agglutination Test for S. aureus58 Slide Agglutination Test for Streptococcus59 Enzyme-Linked Immunosorbent Assay (ELISA)60 Blood GroupingAppendicesAppendix A TablesAppendix B Indicators, Stains, ReagentsAppendix C MediaAppendix D Identification Charts

Benson's Microbiological Applications has been the "gold standard" of microbiology laboratory manuals for over 35 years. This manual has a number of attractive features that resulted in its adoption in universities, colleges, and community colleges. These features include user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. In revising the lab manual for the fourteenth edition, we have tried to maintain the proven strengths of the manual and further enhance it. We have updated the introductory material of the fungi, protozoa, and algae to reflect changes in scientific information. Finally, the names of microorganisms used by the American Type Culture Collection. This is important for those users who rely on the ATCC for a source of cultures.