Laboratory Manual for Microbiology Fundamentals: A Clinical Approach

Laboratory Overview Introduction
Laboratory Safety, Equipment, and Materials
Tools of the Laboratory: Basic Techniques
1 Use of the Microscope
2 Aseptic Techniques
3 Isolation Streak Plate
Eukaryotic Cells and Microorganisms
4 Microbial Phototrophs: Algae and Cyanobacteria
5 Protozoa
6 Fungi
7 Bacterial Motility: Hanging Drop Method
Methods for Microscopic Analysis: Staining
8 Simple Staining and Smear Preparation
9 Negative Stain
10 Capsule Stain
11 Gram Stain
12 Acid-Fast Stain (Ziehl-Neelsen and Kinyoun Methods)
13 Endospore Stain (Schaeffer-Fulton Method)
Methods for Culturing and Diagnosing Microbes: Media
14 Blood Agar
15 Mannitol Salt Agar
16 Eosin Methylene Blue Agar
17 MacConkey Agar
Microbial Nutrition, Ecology, and Growth
18 Nutrients and Growth: Chemical Content of Media
19 Osmotic Pressure and Growth
20 Oxygen and Growth: Aerotolerance
21 Oxygen and Growth: Catalase
22 Oxygen and Growth: Oxidase
Food and Water Safety
23 Plate Count
Treatment and Control of Microbial Growth
24 Killing by Ultraviolet Light
25 Antimicrobial Drug Susceptibility Testing (Kirby-Bauer Method)
Microbial Genetics and Genetic Engineering
26 ELISA and Using Nucleotide BLAST (Dry Lab)
27 Analysis of DNA: Electrophoresis
28 Gene Transfer: Transformation
Infectious Diseases
29 Parasitic Protozoa
30 Parasitic Worms
31 Transmission: Vectors of Disease
32 Transmission: Epidemiology Activity
33 Vaccination and Herd Immunity
Diagnosing Infections
34 Gel Immunoprecipitation (Immunodiffusion)
35 Passive (Indirect) Agglutination
36 Rapid Immunoassays
37 Hemagglutinin Inhibition Assay
38 EnteroPluri-Test
Environmental Microbiology
39 Soil Microbiology
40 Nitrate Reduction
41 Viral Titration
Hydrolytic Enzymes and Disease Mechanisms
42 DNase Test
43 Gelatin Hydrolysis
44 Starch Hydrolysis
45 Casein Hydrolysis
46 Snyder Agar
Biochemical Testing and Diagnosis of Disease
47 Phenol Red Sugar Broth
48 Triple-Sugar Iron Agar
49 Litmus Milk
50 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) Reactions
51 Urease Test
Putting It All Together
52 Identification of Unknown Bacteria

Laboratory Manual for Microbiology Fundamentals: A Clinical Approach is a compilation of decades of experience between the two authors, Steven Obenauf and Susan Finazzo. Their approach will add to your own cadre of techniques for effective teaching, and help your students avoid many of the common pitfalls encountered in the lab. This lab is very visually informative, and has a conversational approach which will entice student interest and facilitate learning and success in your course.